From Bella’s T30 Vial to the Chromatogram

This video ( part 3 of 3 videos of my tour of Analiza Bialek) shows Max preparing my T30 sample from Bella / Nanjing Peptide for HPLC analysis. The sample is reconstituted using purified water and filtered through a 0.22 filter and placed in the newer HPLC system. Max generally uses this instrument for peptides because its thermostated autosampler can cool the samples, allowing them to remain inside safely during overnight testing.

During the analysis, the HPLC separates the sample into its individual components before they pass through the UV detector. A typical run takes around 15 minutes. Max said GLP compounds are the most common peptides he currently receives, with retatrutide probably the most frequently submitted.

The first run of this sample was above the method’s measurement range, so Max needed to dilute or prepare it again and repeat the test. The chromatogram showed one main peak. Although the software displayed names such as tadalafil, Max explained that these were only automatic retention-time matches and did not identify the substance as tadalafil. The relevant information includes the peak area, height and area percentage, which Max then uses for the final calculations.