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Biomass production
gDW/hour
Exchange
Flux
Constraints
(µmol/gDW/hour)
*
Glucose (constrain max influx)
Lactate (constrain max efflux) (90% L-, 10% D-)
Malate (define efflux precisely, influx not allowed)
Glycerol (define precisely)
Glutamine (constrain max influx, efflux not allowed)
Energy
costs
mol. ATP/Haem formed (haemoglobin digestion cost)
mmol. ATP/gDW (maintenance cost)
Biomass composition
% Protein
% DNA
% RNA
% Carbohydrate
% Fat