Citrate (Re)-synthase
| Citrate (Re)-synthase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 2.3.3.3 | ||||||||
| CAS no. | 9077-70-7 | ||||||||
| Databases | |||||||||
| BRENDA | enzyme data | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | enzyme entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| Rhea | reactions | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
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Citrate (Re)-synthase (EC 2.3.3.3) is an enzyme that catalyzes the chemical reaction
The three substrates of this enzyme characterised from Clostridium kluyveri are oxaloacetic acid, acetyl-CoA, and water. Its products are citric acid and coenzyme A.[1] The enzyme was found to have a mechanism dissimilar to that of the more common citrate synthase, since it introduces the acetyl group with stereospecificity for the re face of the oxaloacetic acid.[2][3]
This enzyme belongs to the family of transferases, specifically those acyltransferases that convert acyl groups into alkyl groups on transfer. The systematic name of this enzyme class is acetyl-CoA:oxaloacetate C-acetyltransferase [thioester-hydrolysing, (pro-R)-carboxymethyl-forming]. Other names in common use include (R)-citrate synthase, Re-citrate-synthase, and citrate oxaloacetate-lyase [(pro-3R)-CH2COO→acetyl-CoA].[4]
References
[edit]- ↑ Gottschalk G, Barker HA (1966). "Synthesis of glutamate and citrate by Clostridium kluyveri. A new type of citrate synthase". Biochemistry. 5 (4): 1125–33. doi:10.1021/bi00868a003. PMID 5958189.
- ↑ Dittbrenner S, Chowdhury AA, Gottschalk G (1969). "The stereospecificity of the (R)-citrates synthase in the presence of p-chloromercuribenzoate". Biochem. Biophys. Res. Commun. 36 (5): 802–8. Bibcode:1969BBRC...36..802D. doi:10.1016/0006-291X(69)90680-9. PMID 5808294.
- ↑ Gottschalk G (1969). "Partial purification and some properties of the (R)-citrate synthase from Clostridium acidi-urici". Eur. J. Biochem. 7 (2): 301–6. doi:10.1111/j.1432-1033.1969.tb19607.x. PMID 4974734.
- ↑ Enzyme 2.3.3.3 at KEGG Pathway Database.
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